Background: Stroke is the second most prevalent cause of mortality worldwide and a primary contributor to disability, with a rising occurrence in emerging nations. The association between serum lipids and ischemic stroke is still a subject of debate. Research on lipid-related risk factors in cerebrovascular disease has shown inconsistent results and has used different definitions for the cerebrovascular endpoints. So, the current study aimed to evaluate the significance of serum lipid profile as a risk factor in ischemic stroke. Materials and Methods:Present study was conducted in Department of General Medicine, Government Medical College, Kannauj, Uttar Pradesh, India.A cohort of 60 patients diagnosed with ischemic stroke and 60 healthy individuals who attended regular medical examinations were included in the study during July 2023 to December 2023. Comprehensive demographic and clinical information of all the patients was acquired. The stroke diagnosis was verified with a cerebral CT scan. A lipid profile was conducted on the day after admission, following an8-hour fasting period. Data was examined utilizing the SPSS program. Results:An evident discrepancy was noted when comparing the levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) between the stroke patients and the control group. Moreover, while analyzing dyslipidemia, it was apparent that the group of patients who had experienced an ischemic stroke had a much higher percentage of individuals with dyslipidemia in comparison to the control group. Conclusion:It is important to consider the lipid profile assessment when calculating the individual risk of stroke.