Background: Lumbar disc prolapse (LDP) is a common condition that can lead to significant morbidity. Genetic factors, including variations in the COL9A2 gene, have been implicated in the susceptibility to disc degeneration. This study aims to investigate the association between COL9A2 gene variants in a middle part of the Indian population. Methods: 100 LDP patients and 100 healthy controls participated in a comparative study. Restrictions fragment length polymorphism (RFLP) analysis and polymerase chain reaction (PCR) were used in the genotyping of COL9A2 variants rs2070873 and rs144520236. Using chi-square tests, the genotype and allele frequencies were compared between the groups to assess statistical significance. Results: The frequency of the A allele of rs2070873 was significantly lower in LDP patients (66%) compared to healthy controls (74%) with a p-value of 0.04, indicating a potential protective effect of this allele against LDP. No statistically significant association was found for the rs144520236 variant (p=0.1). Conclusion: The findings suggest that the COL9A2 rs2070873 variant is associated with a decreased risk of LDP in the studied population. This underscores the importance of genetic factors in the pathogenesis of LDP and highlights the potential for genetic screening in identifying at-risk individuals.