The effectiveness of the treatment of Iron Deficiency Anemia (IDA) in rats using Freeze-dried Crocodile Blood (FCB) was performed by using 10 groups of 5, male, Sprague-Dawley rats. After 4 weeks of IDA induction (groups G2-G10), the rat’s tail-vein blood was collected for hematological and serum protein investigations. The induced groups hemoglobin (5.59±0.25 g/dl) and hematocrit (26.46±6.45%) were significantly different (p<0.05) from the control group (G1). Rats (G2-G8) were treated with FCB in various doses as design for 24 weeks. Group G6 fed FCB 500 mg and vitamin C 50 mg was as effective as groups G4-G5, showed hemoglobin (12.20±0.82 gm/dl) and hematocrit (33.06±2.09%) significantly different (p<0.05) from groups G2-G3. These revealed that the FCB and low dose of vitamin C was efficient when used to increase hemoglobin and hematocrit values. After treatments, the FCB groups had no detrimental effect on internal organs - with the exception of group G10 fed with AIN93G-Fe diet and 60 mg ferrous sulfate per day. The serum protein analysis demonstrated an alpha-1 proteinase inhibitor (alpha-1-inhibitor III) was released in high volume; depend on FCB treatment dose. Furthermore, haptoglobin and preprohapto-globin from the IDA rats were released in higher volumes than G1 or FCB groups.