Background: Antibiotic resistance is a growing global concern, exacerbated by the inappropriate use of antibiotics. Endodontic procedures often necessitate antibiotic prescriptions, and understanding the knowledge, attitude, and practices (KAP) of dental practitioners regarding these prescriptions is crucial for promoting rational antibiotic use. This study aims to evaluate the antibiotic prescription patterns among dental professionals in India during endodontic procedures. Materials and Methods: A cross-sectional KAP survey was conducted among 500 dental practitioners across various regions of India. A structured, self-administered questionnaire was designed to assess their knowledge, attitudes, and practices related to antibiotic prescription in endodontic treatments. The questionnaire comprised 25 questions covering demographics, clinical scenarios, antibiotic selection, duration of prescription, and awareness of antibiotic resistance. Data were analyzed using descriptive statistics, chi-square tests, and logistic regression models to identify factors influencing antibiotic prescribing behaviors. Results: Out of 500 respondents, 60% were male and 40% were female, with an average age of 35 years. Approximately 70% of the practitioners demonstrated adequate knowledge regarding antibiotic use in endodontics. However, 45% admitted to prescribing antibiotics unnecessarily due to patient pressure or uncertainty in diagnosis. The most commonly prescribed antibiotics were amoxicillin (80%), followed by metronidazole (15%) and clindamycin (5%). Nearly 50% of practitioners prescribed antibiotics for a duration longer than recommended guidelines. A significant association was found between years of practice and the likelihood of inappropriate antibiotic prescribing (p<0.05). Conclusion: The study reveals a discrepancy between knowledge and practice among Indian dental practitioners regarding antibiotic prescriptions in endodontic procedures. Despite good knowledge, the influence of external factors leads to inappropriate prescribing practices. Interventions such as continuing education programs and strict adherence to clinical guidelines are essential to promote rational antibiotic use and curb antibiotic resistance.