Jatropha curcas L. and Ricinus communis L. belong to Euphorbiaceae and considered as important alternative biofuel. Somatic hybridization between J. curcas L. and R. communis L. was proposed to solve low genetic variability of J. curcas L. The intergeneric hybrid will provide a novel variety which perform as an annual crop and bear fruits to ease harvesting for commercial production. Protoplast fusion between J. curcas L. and R. communis L. to create intergeneric hybrid was attempted via PEG-mediated method. Mesophyll protoplasts of J. curcas L. and calli protoplasts of R. communis L. were used in this study. Concentration and molecular weight of PEG, fusion period, microfusion and macrofusion method were optimized. The highest (66%) viability of heterokaryon was obtained from using 30% PEG (MW6000) assisted with macrofusion method for 10 minutes.