Objective: The present study was conducted for comparing the efficacy of conventional needle irrigation, EndoVac, and EndoActivator in removing smear layer and organic debris from the root canal. Materials and methods: This study involved the use of 60 single-rooted human teeth without prior endodontic treatment and intact apices. Teeth with extensive restorations, root caries, fractures, immature apex, and root length shorter than 10 mm were excluded. Smear layer was assessed and results were evaluated. Results: The EndoActivator demonstrated superior performance in total smear layer and debris removal compared to the control group treated with saline solution, which showed the least favorable outcomes. Conclusion: The EndoVac system and the EndoActivator system showed significantly greater effectiveness in cleaning root canal walls compared to conventional needle irrigation methods.