Background: Adverse drug reactions (ADRs) with anti-tubercular treatment (ATT) are associated with substantial morbidity.Ready reckoner on management of ADRs with ATT was released by Govt of India for guidance of medical officers (MOs). Studies on evaluation of adherence on healthcare policies and guidelines are scanty in literature. It was planned to evaluate knowledge of management of ADRs with ATT among MOs in a tertiary hospital as per the Ready Reckoner. Methods: This was an observational and questionnaire-based survey designed from Ready reckoner involving 200 MOs.The score was noted based on the comments given on the questionnaire by the physicians. Total score: 10, Excellent knowledge: 08-10, Good knowledge: 07-05, Poor knowledge: <5. Results: Only 37.5% of MOs were having awareness about Ready Recknor.However, 73% of MOs were having excellent and good knowledge about management of ADRs with ATT. Poor knowledge was noted in 27% of MOs.Significant numbers of junior residents (JRs) from paraclinicaldepts had poor knowledge (43%) compared to JRs from clinical depts. Conclusion: The results of study emphasize the need for healthcareguideline awareness by MOs developed by Government by implementing measures like additional training and easy access to these guidelines. The healthcare policies and guidelines established by the Government and prominent academic bodies of India should be incorporated into the MBBS curriculum so that they are thoroughly acquainted with them.