Background: Candida species like Candida albicans and non candida albicans are the major cause of vulvo vaginal candiasis (VVC). These organisms produce an array of virulence factors which makes it to initiate the infection. The present study is undertaken to study the fungal causative agents, their species distribution and virulence factors from the Candida species isolated from the cases of VVC from a tertiary care hospital. Methods: All female patients in the reproductive age group (18-49 years) fulfilling the clinical criteria of are included in the study. Phenotypic characterisation was done by Gram stain morphology, KOH wet mount examination and culture on Sabouraud’s dextrose medium. Species differentiation determined by growth on CHROM agar and sugar assimilation tests. Virulence factors like formation of biofilm, proteinase and phospholipase are tested by standard methods. Result: A total number of 116 (33.1%) subjects showed pure growth of Candida species by conventional standard methods. The most common species isolated by conventional methods was C.albicans (36.3%) followed by C. glabrata (24.1%), C. tropicalis (22.5%), C. krusei (10.3) and C.parapsilosis (7.7%). Among biofilm, proteinase and phospholipase producers, it was seen that C. albicans (46.5%, 48.1% & 41.8% respectively) was the commonest producer. Followed by C. glabrata, the commonest biofilm, proteinase & phospholipase producer was (23.2%, 23.4 & 25.4% respectively). Conclusion: Identification and species differentiation is essential for the management of cases with VVC. Virulence factors were produced in significant amount by the isolated strains of Candida species.