Background:The foramen Vesalius and the canaliculus innominatus are two anatomical structures found in the human skull. The present study was conducted to assess presence of Foramen Vesalius and Canaliculus Innominatus. Materials & Methods:40 human skullsof both genders were assessed for the presence of FV and CI in middle cranial fossa and the incidence (unilateral and bilateral) was noted. Distance of FV from the ipsilateral FO was measured as well. Shapes of these foramina were also recorded. Results: Diameter of Foramen Versalius on right side was 0.8mm, on left side was 0.6 mm, distance from foramen oval e was 4.1 mm on right side and 3.8 mm on left side. Shape was round in 22 and 18, irregular in 17 and 20 and oval in 1 and 2 on right and left side respectively. The difference was significant (P< 0.05). Diameter of Canaliculus Innominatuson right side was 1.8 mm, on left side was 1.9 mm, distance from foramen oval e was 1.5 mm on right side and 1.4 mm on left side. Shape was round in 21 and 15, irregular in 5 and 6 and oval in 3 and 1 on right and left side respectively. The difference was significant (P< 0.05). Conclusion: The results of this study may be significant to anatomists as well as physicians who handle the middle cerebral cavity in the course of different procedures.