Background: This study was conducted for evaluation of serum lipid profile among gallstone patients. Material and methods:In total, 100 subjects participated in this study, who were split into two groups: group 1 included 50 healthy controls and group 2 included 50 subjects with gallstones. An illustration of the results included the mean and standard deviation. Lipid profile was assessed and compared.The data was compared between the subjects and healthy persons using the Student's t-test. Results: In this study there were 100 subjects which were divided into 2 groups of 50 each. Group 1 comprised of 50 subjects with gallstones and Group 2 comprised of 50 controls. Serum cholesterol levels were higher among those having gallstones (172.56) as compared to controls (165.98). In contrast to the controls (189.11), individuals with gallstones (193.23) had higher serum triglyceride concentrations, and the analysis was shown to be statistically significant. There was decrease in the blood HDL levels among gallstone-afflicted participants (18.59) as compared to the control group (25.66). However, compared to the control group (130.26), it was discovered that the gallstone patients (121.36) had lower serum LDL levels. Conclusion: It had been concluded that serum triglyceride quantities as well as serum HDL amounts had been statistically significant among gallstone subjects as well as there existed a positive association among these parameters as well as gallstone disease.