Acute leukemia is the most common pediatric hematological malignancy. Bloodstream infections (BSIs) are severe complications in these patients during chemotherapy. This study Microbiological characteristics of BSIs in Children of hematologic malignancy. All Pediatric patients of Hematological Malignancy aged < 18 years, were include in the study. Study was conducted in Department of Pediatrics and Microbiology of a tertiary care Hospital. Study duration was 1yr from August 2021 to July 2022 after approval from Institutional Ethics committee.A total of 117nonduplicate different samples were collected from 100 patients and processed for bacteriological culture. A total of 33 bacterial isolates were grown.Pseudomonas and Staphylococcus were most common isolates from blood and E.coli from Urine specimens.