Neonatal sepsis is defined as a blood stream infection whichdevelops within28days afterbirthor up tofour weeks. It is an important cause of mortality among neonates. Basedonthe time of onset, it is categorized into early onset neonatal sepsis (EONS) and late onset neonatal sepsis (LONS). It encompasses systemic infection of the new born including septicemia, meningitis, pneumonia, arthritis, osteomyelitis, congenital syphilis and urinary tract infection. In This study blood sample from neonate with 2 or more risk factor suggestive of EONSwas collected from cord blood and Peripheral vein. A total of 100 blood samples were collected for culture . In 65.7% cases clinically significant with sepsis screen positive EONS was found using cord blood and 60% using peripheral veinblood . Klebsiella spp(20.8%)was found to be most common isolates followed by E.coli(16.7%), Staphylococcus aureus (16.7%), Pseudomonas spp(12.5%) and Enterococcus spp(12.5%).Finding suggestive of Cord blood superiority compare to peripheral vein blood in finding causative agent of EONS.