Aim: To determine the prevalence of Methicillin-Resistant Staphylococcus aureus (MRSA) and identify the risk factors associated with wound infection in patients. Material and methods: Cross- sectional research was conducted in the department of microbiology ASMC Etah and FH medical college to evaluate the antibiotic resistance pattern of methicillin-resistant Staphylococcus aureus that was isolated from wound infections, as well as the risk factors associated with it. This research included all patients who were suspected of having wound infections and had not received any antibiotics during the two weeks before the study period. A total of 100 participants were included in this trial. Data pertaining to sociodemographic characteristics and their corresponding risk factors were gathered via the use of a well-organized questionnaire. Results: Among a group of 100 patients who were suspected of developing wound infection, 50 of them (50%) were found to have S. aureus wound infections based on culture confirmation. Out of them, 15 (30%) were MRSA. The study population had an overall prevalence rate of 15% for MRSA, with 15 out of 100 individuals affected. Out of the 31 patients who were admitted to the hospital and the 69 patients who were treated as outpatients and suspected of having a wound infection, 38.71% (12 out of 31) and 33.33% (23 out of 69) were found to have a positive culture for S. aureus, respectively. The total incidence of Methicillin-resistant Staphylococcus aureus (MRSA) in hospitalized patients was 22.58% (7 out of 31), whereas in non-hospitalized patients it was 11.59% (8 out of 69). Conclusion: Among the 100 patients who were suspected to have wound infection, 50 of them, which is 50% of the total, were confirmed to have S. aureus by culture testing. Out of them, 15 (30%) were MRSA. Wound infection caused by MRSA had a significant correlation with profession, being diagnosed in the inpatient department, and body mass index. More than 50% of MRSA isolates exhibited resistance to gentamicin, ciprofloxacillin, cotrimoxazole, and erythromycin.