Background: Antibiotic resistance specially in the form of metallobetalactamase production (MBL) in gram negative bacilli is major health concern and present a major therapeutic challenge nowadays. In this study, phenotypic confirmation of various methods for detection of MBL is done by various methods. Material& Methods: The isolates were screened for metallo-beta-lacta¬mase production by using imipenem. All the imipenem resistant isolates were further subjected to a phenotypic confirmation by the combined disk synergy test, modified Hodge test and Vitek 2 system. The results were analyzed and tabulated. Results: 63% of our isolates were MDR organisms. Of 260 isolates, 68 were found to be carbapenem resistant. A total of 34 of 37 (91.8%) A. baumannii isolates were carbapenem resistant.. In case of Enterobacterales only 29 of 119 (24.3%) and 5 of 8 (62.5%) P. aeruginosa were carbapenem resistant. Many of these isolates were resistant to multiple antimicrobial drugs with the highest resistance shown by carbapenem resistant isolates of A. baumannii. The majority of the strains were sensitive to colistin and polymyxin B. In our study, MHT for detection of carbapenemases was positive in 76% (52/68) of carbapenem resistant isolates. This study also depicts that Class B carbapenemases i.e. MBL can be detected by Vitek as compared to CDST and VItek. Conclusions: Carbapenem resistance has been found to be widespread. This emergence might outcome from the absence of public health surveillance programs in most countries. So the public health surveillance programs must be set up in all countries to discover these problems as early as possible.