Background: The present study was conducted for assessing the change in serum cytokine levels among patients undergoing general anesthesia. Materials & methods: A total of 20 patients of ASA grade I/II were enrolled. Complete demographic and clinical details of all the patients was obtained. Only those patients were enrolled which were scheduled to undergo surgeries under general anesthesia. Preoperatively, blood samples were obtained. Serum cytokine levels were recorded. Anesthesia was induced with thiopental and succinylcholine, and endotracheal intubation was performed. Serum blood samples were obtained again and serum cytokine levels were evaluated. All the results were analyzed using SPSS software. Results:Mean IL-6 levels before surgery and after surgery was 2.39 pg/ml and 6.21 mg/ml respectively. Significant results were obtained while comparing IL-6 levels before and after surgery (p-value < 0.05). Mean IL-10 levels before surgery and after surgery was 3.23 pg/ml and 3.85 mg/ml respectively. Non-significant results were obtained while comparing IL-6 levels before and after surgery. Conclusion:Significant alteration of cytokine levels occurs among patients undergoing general anesthesia.