Introduction:HealthCare facilities can be sources of pathogenic bacteria that can lead to hospital-acquired infections (HAIs).Bacteria, mould, and viruses can grow on ventilation system, as well as on the moist ceiling and floor.The airborne transmissions are also more prevalent in healthcare surroundings due to overburdened hospitals and the presence of immune-suppressed patients. Methods:An institutional-based cross-sectional study design was used to assess the bacterial & fungal load in the indoor (one random sample) and outdoor patients’ (before starting OPD & during OPD) area of the hospital. To determine the bacterial load, a passive air sampling technique was used. The settle plate method was used to collect datawhich involved exposing blood agar and sabouraud’s dextrose agar (SDA) media to OPD & IPD rooms for 1hour. Results: The bacterial and fungal load was comparatively less before starting the OPDs. The area of windows and doors’ are less compared to total room volume. The bacterial load in the air found to be >1000 CFU/mm3in OPDduring OPD hours and IPD areas. The non-sporinggram-positive bacilli (GPB-NS) were the most common isolated microorganism followed by gram positive cocci (GPC) and Aspergillus species. Conclusion:The majority of the wards in the present hospital had bacterial loads in the air that exceeded WHO guidelines. Over-crowding, high temperatures, inadequate ventilation couldcontribute to a high concentration of bacteria in the indoor air. Strict visitors and infection control policy as well as environmental,construction audit is needed for infection prevention and control measures.