Background: As people live longer, there are more elderly people who need rehabilitation due to periodontal disease and tooth caries, which makes them edentulous. The present study was conducted to assess effect of abutment types and loading protocol on success of dental implant. Materials & Methods: 54 partially edentulous patients of both genders were divided into 2 groups of 27 each. Group I was in which delayed loading and group II was in which immediate loading was done. Probing pocket depth, bleeding on probing, marginal bone loss, and coronal height of soft-tissue were recorded at baseline, 1 year and 2 years. Quantitative PCR was done to detect interleukin-1beta (IL-1β), tumor necrosis factor-alpha (TNF-α), osteocalcin (OC), alkaline phosphatase (ALP), tartrate-resistant acid phosphatase (TRAP). Results: The mean marginal bone loss at baseline, 1 year and 2 years at implant level in group I was 1.51, 0.20 and 0.41 and in group II was 1.64, 0.22 and 0.13. At oxidized abutment was 1.07, 0.56 and 0.92 in group I and 1.31, 0.41 and 0.28 in group II. At milled abutment site was 1.07, 0.20 and 0.12 in group I and 0.96, 0.35 and 0.28 in group II. The difference was significant (P< 0.05). At baseline, 1 year and 2 years in group I and II, the mean plaque score was 0.98, 1.12, and 1.60 in group I and 0.97, 1.11, and 1.61 in group II. Probing depth was 1.09, 0.19, and 0.12 in group I and 0.96, 0.42, and 0.28 in group II. Mucosal bleeding was (%) was 28.1, 35.2, and 38.5 in group I and 30.1, 36.2 and 38.0 in group II. Bleeding on probing (buccal) was 10.4, 12.3, and 14.9 in group I and 11.6, 13.2, and 15.8 in group II. Bleeding on probing (proximal) was 10.8, 11.5, and 16.4 in group I and 10.6, 11.3, and 15.9 in group II. Soft-tissue height was 1.9, 2.5, and 2.1 in group I and 1.8, 2.4, and 2.2 in group II. The difference was significant (P< 0.05). The mean TNF-α value at 3 days, 1 month and 3 months was 46.2, 11.6 and 15.9 in group I and 97.2, 11.6, and 15.2 in group II. TRAP was 3.8, 2.1, and 13.2 in group I and 2.7, 1.2, and 1.1 in group II. IL-1β value was 972.4, 432.6, and 575.4 in group I and 11.8, 250.4, and 296.4 in group II. Alkaline phosphatase level was 11.6, 6.2, and 25.9 in group I and 32.7, 11.4, and 4.8 in group II. Osteocalcin level was 1.5, 3.2, and 42.8 in group I and 2.7, 1.2, and 2.9 in group II. The difference was significant (P< 0.05). Conclusion: In terms of soft-tissue characteristics, biomarkers in sulcular fluids, and bone loss, both immediate loading and delayed loading implants exhibit comparable outcomes with comparatively few and controllable problems.