Aim: The purpose of this research is to investigate and analyze the impact of calcium sulphate that has been supplemented with antibiotics in orthopaedic therapy. Materials and Methods: 50 Individuals who had been previously diagnosed with osteomyelitis, peri-prosthetic infection, or open fracture were invited to participate in the research. After obtaining the necessary permission, the participants in the research were recruited. The patients' clinical histories were recorded, and they were evaluated; preoperative laboratory values (TC, ESR, and CRP) were obtained; and radiographic examinations were carried out. Patients were evaluated using laboratory parameters (TC, ESR, and CRP), radiographs in anteroposterior and lateral views for resorption of calcium sulphate and bone healing, which comprised the basis line of the research. Patients were followed up-to 8 weeks, 4 months, and 8 months postoperatively. Results: 50 cases were included in our research, and each one was categorized into a specific type based on the ailment. There were 10 cases of osteomyelitis, 25 cases of periprosthetic fracture, 13 cases of open fracture, and 2 cases of de-gloving injury to the left leg. All of the cases were grouped together into our study. Each patient was given a preoperative evaluation before undergoing the surgical procedure, which consisted of the administration of one gram of vancomycin-impregnated calcium sulfate pellets. Eight months of follow-up were performed on each of the patients. In 45 of the instances, we had successful outcomes. 5 of the cases were deemed unsuccessful. In cases of osteomyelitis, peri-prosthetic infection, and open fracture, laboratory parameters with statistically significant P values were observed in TC, ESR, and CRP as follows: osteomyelitis (0.01, 0.01, 0.001) Peri-prosthetic infection (0.001, 0.001, 0.001) and in open fractures(0.001, 0,002,0.001) were noted. Conclusion: When applied topically at the site of an infection, antibiotic-impregnated calcium sulfate promotes bone healing and plays an important part in the fight against the infection, according to the findings of the research that we carried out.