Background: Health care-associated infections (HCAIs) are those infections that patients acquire while receiving health care. Around 8.7% of the patients in the hospitals fall under the risk of exposure to the nosocomial infections which multiply the complicated conditions such as cancer, surgery, or any cases of organ transplant, thereby surging the mortality rate. The present study was conducted for the Evaluation of hospital acquired infections among patients admitted in wards. Material and methods: A total of 500 patients admitted to medicine and surgery ward were enrolled in the current research. Inclusion criteria for the present study included patients that were admitted in the department for various procedures, without evidence of initial infection. Pus, blood, urine, sputum and swabs from various lesions if present among study patients was taken after 48 hour of admission and followed till discharge from the hospital. Identification of bacterial strain was done with help of various biochemical tests. All the results were recorded and analysed using SPSS software. Results: A total of 500 subjects were screened. Mean age of the patients was 51.8 years. Out of these 500 patients, nosocomial infection was seen in 19 percent of the patients. Enterococcus faecalis was seen in 80 percent of the cases with NI while Acinetobacter spp. was seen in 75.79 % of the cases with NI. Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella species were identified in 45.26 %, 40 %, 32.63 % and 26.32 % of the cases with HCAIs. Conclusion: Because of the rising prevalence of HCAIs in hospitals, greater attention needs to be paid to cleaning the wards.