Background: This research had beencarried out to evaluate the function of trace elements in oral submucous fibrosis subjects. Material and methods:There were 100 participants in all, 50 of whom were controls and 50 of whom had an OSMF diagnosis. Every patient had OSMF, which was verified by histological examination. A computerized autoanalyzer photometer was employed to examine blood samples obtained from 100 distinct individuals. This colorimetric study was performed to determine the blood's copper content. We employed statistical analysis to compare the case and control outcomes. Results: It was noticed that the levels of copper were reduced in the control cohort and high in study cohort. Conclusion:The most accurate method for diagnosing Oral Submucous Fibrosis (OSMF) is a biopsy; however, this procedure is also the most invasive and time-consuming. Many contemporary advancements are currently being employed to detect OSMF early and stop it from advancing to advanced and irreversible stages. Trace element testing may be helpful for patients with oral submucous fibrosis (OSMF) as a diagnostic and prognostic method.