Background:Vitamin D plays a vital role in mediating various metabolisms which include bone and cell growth. It binds with the vitamin D receptor (VDR) and regulates calcium homeostasis. It promotes cell differentiation. The converse correlation between vitamin D and the development of breast cancer was demonstrated in various studies. Breast cancer is also considered the leading cause of mortality among women around the world and its incidence is annually increasing. Several studies were conducted to determine its association with VDR. Aim: To determine the contribution of the VDR polymorphism to breast cancer risk in patients and the control group.Methods:Genomic DNA was isolated from blood samples of 100 women with breast cancer and 100 healthy women. After the amplification of five positions of VDR gene, the prepared amplicons were digested with BsmI, FokI, TaqI, ApaI, and poly A restriction enzymes.Result:Subsequently, the digested products were electrophoresed on the 2 % agarose gel. Odds ratios (ORs) for breast cancer were calculated for genotypes and estimated haplotypes. The distribution of VDR BsmI (rs1544410), FokI (rs2228570) and ApaI (rs7975232) polymorphisms was more significant among the patients than to the normal group. Analysis of linkage disequilibrium for all pairs of SNPs showed the significance between BsmI and TaqI. The major haplotypes of BsmI, FokI, ApaI, TaqI and PolyA SNPs were significantly in high frequency.Conclusion:Our results showed that BsmI, FokI and ApaI of VDR polymorphisms are associated with the risk of breast cancer progression.