Background:The present study was conducted for assessing serum vitamin D level sin diabetic patients. Materials & methods:A total of 100 patients with type 2 diabetes and 100 healthy controls were enrolled. Complete demographic and clinical details of all the patients was obtained. Inclusion criteria for the study group included whose diabetes was confirmed and were between 30-60 years old and agreed to participate in this study. Blood samples were obtained from all the patients and was sent to laboratory where Vitamin D levels were evaluated using an auto-analyzer. Vitamin D deficiency was defined as serum 25(OH) D concentration of less than 20ng/ml, insufficiency as 20ng/ml <25(OH) D<30 ng/ml and sufficiency was defined as 25(OH) D higher than 30 ng/ml. all the results were recorded in Microsoft excel sheet and was subjected to statistical analysis. Results: Mean vitamin D levels among the patients of the study group and control group was 16.3 ng/ml and 23.1 ng/ml respectively. While comparing the results, significant results were obtained. Conclusion:Vitamin D levels are significantly affected in diabetes patients.