Background: Thepresent study was conducted for Comparing the Accuracy of Two Different Impression Materials in Making Duplicate Dies. Material and methods:The study used two impression materials: Panasil and Speedex.Making duplicate dies. The institutional ethical committee provided prior approval.This study used a step-by-step impression procedure, followed by the pouring of dental stone.Stone casts were removed from the imprint and preserved until final setting. Ten impressions were made, five for each impression substance. Die fabrication was completed. The marginal discrepancy was measured on the mesial, distal, buccal, and lingual sides using the specified technique. Results: Two groups were formed. The first group comprised of panasil impression material (Group I) while the 2nd group comprised of speedex impression material(Group I). A significant difference was observed on buccal side in the two groups whereas on lingual side in group I. Substantial variationwas also observed on mesial side in group I whereas on distal side in group II. Conclusions:Panasil outperformed Speedex for die duplication accuracy.