Purpose: Urinary tract infection patients are more likely to have vancomycin-resistant enterococci in a hospital setting. The present study was undertaken to isolate and identify enterococci from urine sample by standard technique, to detect prevalence of Vancomycin Resistant Enterococci, to detect antibiotic profile with Urinary Tract Infection caused by Vancomycin Resistant Enterococciand to identify risk factors associated with Urinary Tract Infection caused by Vancomycin Resistant Enterococci. Materials and Methods: The study comprised of 50 isolates of Enterococcusspp isolated from patient’s urine suffering from Urinary Tract Infection using standard microbiological procedures. Antibiotic susceptibility testing by Kirby Bauer Disc Diffusion Method was performed using antibiotics as per CLSI guidelines. MIC of Vancomycin was determined by E-Test. Results: Out of 50 Enterococcus isolates the highest prevalence of Enterococcus species from urine sample was shown by Enterococcus faecalis (76%) than Enterococcus faecium (24%). The prevalence of Vancomycin Resistant Enterococci observed by E- test was greater in E. faecium (33.33%) in compared to E. faecalis (21.04%). The major risk factor for VRE colonization in UTI patient was found to be comorbidities i.e.33.33% followed by advanced age (25%), exposure to ICU (16.66%), patient with catheter (16.66) and patient on HD (8.33%) respectively. Conclusion: E. faecalis and E. faecium were the major enterococcal strain which are major pathogen of urinary tract infection. The prevalence of Vancomycin Resistant Observed by E. test in E. faecium (33.33%) was greater in compared to E. faecalis (21.04%).