Background: The purpose of this study was to use the Drager jaundice metre JM-105 to compare transcutaneous bilirubin (TcB) measurement with total serum bilirubin (TSB) in neonates with clinically significant jaundice.Methods: A tertiary care hospital carried out a cross-sectional study on 50 jaundiced neonates who needed their serum bilirubin levels to be determined between days two and three of life.Results: The transcutaneous bilirubin (TcB), which is tested at the forehead, was 14.1 mg/dL, while the mean total serum bilirubin (TSB) was 14.4 mg/dL. Additionally, the mean difference between TcB and TSB was 0.3 mg/dL. Between TSB and TcB forehead, a positive linear connection was found (r= 0.76).Conclusion: Because of its accuracy, TcB measured at the forehead is a useful non-invasive diagnostic test for screening newborns from South India for hyperbilirubinemia.