Background:To study the prevalence of asymptomatic bacteriuria in pregnancy, for its early screening, diagnosis, and appropriate treatment. Materials & Methods:The present study was conducted on antenatal patients in the Department of Obstetrics and Gynecology and Microbiology of Dr. B.L. Kapur Memorial Hospital, Pusa Road, New Delhi.Methods for diagnosing ASB include midstream urine culture (the gold standard), Gram stain and urine dipstick tests. A urine culture can take up to two days to get a result, with the threshold for diagnosis usually defined as the presence of 105 colony-forming units (CFU)/mL of a single organism. Data collected during the study was tabulated in Microsoft Excel. Statistical analysis wasconducted with the statistical package for the social science software version (SPSS 20.0). The quantitative data were processed by Student t test and ANOVA and qualitative measurements by Chi-square test. For all statistical tests, a p value less than 0.05 was taken to indicate a significant difference/association. Results:Overall prevalence of asymptomatic bacteriuria was observed to be 6.21% (9/145) among the pregnant females.In present study, urine samples were collected in all the three trimesters for screening from all the subjects. Means and standard deviations of gestational periods (POG) at the time of screening were 10.7±1.16, 20.7±2.27 and 32.2±2.17 weeks in first, second and third trimester respectively. A subject showing pus cells ≥10/HPF in any trimester during her pregnancy was considered as having a positive urine routine microscopy test. Of the 145 total subjects, 68 subjects (46.90%) were positive for this test. Conclusion:The most common uropathological isolate found in our study was Escherichia coli (88.9%) followed by Klebsiella (11.1%).Complications related to asymptomatic bacteriuria occur more profoundly if left untreated to reach the stage of pyelonephritis. All the sequelae of ASB during pregnancy can be reduced by early detectionand appropriate antimicrobial treatment. Hence, it should be made mandatory to screen every antenatal women in each trimester, to save bigger complications at lesser cost.