A Tertiary Care Hospital Study in Madhya Pradesh delves into the evolving landscape of Human Immunodeficiency Virus (HIV) and Acquired Immune Deficiency Syndrome (AIDS) in India, exploring patterns, prevalence, and associated risk factors. Despite a 66% reduction in new infections since 2000, India grapples with the second-highest global HIV burden. This study highlight burden of HIV in our region among different age group associated with sexual practices and high risc behavior responsible for transmission of HIV. A significant no of HIV positives were found in this study 490 in different groups, highest number of positives were from Heterosexual group of individuals.