Background: Chronic wound infections, exacerbated by microbial pathogens such as bacteria and fungi, pose a significant contemporary healthcare challenge. These infections are further complicated by the escalating problem of antimicrobial resistance. Natural compounds, such as essential oils (EOs), comprised of a complex mixture of components, emerge as a potential solution. However, the landscape is clouded by concerns about the efficacy and safety of non-controlled commercial essential oils. Methods: In this study, the commercialized Cinnamomum zeylanicum EO in Tunisia were nalysed using GC/FID and GC/MS and tested aginst 13 bacterial strains responsible for skin infectionsusing the disc diffusion and microdilution broth methods. Results: Eighty components, representing 96.54% of the total oil, were identified. The predominant component was the aldehyde (E)-cinnamic aldehyde (66.23% ± 2.010%), followed by the sesquiterpene hydrocarbons β-caryophyllene (6.43 ± 0.73%), the monoterpenic alcohol linalool (3.58 ± 0.26%), and the monoterpenic ester cinnamyl acetate (3.14 ± 0.18%).The antibacterial activity of the EO varied significantly among microbial strains and methods used. The highest antibacterial activity was observed against the Gram-positive bacterium Staphylococcus aureus ATCC 2912, followed by the Gram-negative Proteus mirabilis P195/20, with an inhibition zone diameter higher than that produced by the antibiotic Gentamicin. Conclusions: Our findings suggest that C. zeylanicum EO could be considered as an alternative treatment for skin infections, given its promising antibacterial and activities.