Background: Pleural infection is a clinical problem that affects all ages but is most common in children and elderly patients.sterile body areas Patients may experience severe morbidity and mortality if they become contaminated with germs. The current study is intended to identify the bacterial profile and trend of antibiotic resistance associated with pleural fluid infection in a tertiary care centre. Material method: Using aseptic measures, a thoracocentesis was used to obtain pleural fluid samples. The study comprised all specimens of pleural fluid from July 2023 to December 2023 from the departments of medicine, paediatrics, and TB Chest for Culture and Sensitivity to Microbiology. Result: Total sample of Pleural fluid received from July 2023 to December 2023 were 495 out of which 70 (14.2%) showed growth while 425 (85.8%) samples were sterile. Majority of isolates were Gram negative bacilli 77 %. Conclusion: Antimicrobial resistance has increased in both gram positive and gram negative bacteria as a result of the careless use of antibiotics. Patients' knowledge of the harmful effects of antibiotic overuse and misuse must be raised, and empirical treatment should be promoted.