Background: The present study was conducted for analysing and comparing preoperative and postoperative Serum Lipid Profile in Patients with Cholelithiasis undergoing laparoscopic cholecystectomy. Materials & methods: A total of 200 patients were enrolled. Complete demographic and clinical details were recorded. Preoperative radiographic findings were evaluated separately. Blood samples were obtained and preoperative serum lipid profile was assessed. All the patients underwent laparoscopic cholecystectomy. Follow-up was done and lipid profile was evaluated again. All the results were recorded in Microsoft excel sheet followed by subjected to statistical analysis using SPSS software. Results: Mean total cholesterol, triglycerides, HDL and LDL during preoperative time interval was 184 mg%, 186.5 mg%, 41.3 mg% and 105.4 mg% respectively. Mean total cholesterol, triglycerides, HDL and LDL during postoperative time interval was 195.8 mg%, 215.8 mg%, 43.8 mg% and 108.9 mg% respectively. Conclusion: Lipid profile is significantly altered among cholelithiasis patients undergoing laparoscopic cholecystectomy.