Introduction: Omeprazole is a proton pump inhibitor used for acid-related disorders. It exerts an anti-inflammatory effect owing to an inhibition of v-type H+ K+ ATPase located on neutrophils thus interfering with the neutrophil function that contributes to inflammation. Previous studies demonstrated omeprazole anti-inflammatory action. This study was planned to evaluate the analgesic action of omeprazole. Material and methods: Eighteen albino rats (Wistar strain) of either gender (weight 100-200 g) screened for gross anomaly and divided into 3 random groups of 6 animals each. Groups I, II, and III received omeprazole (10 mg/kg, 20 mg/kg, and 40 mg/kg, per oral each, respectively). The animals in each group served as their own control. Tests used were Haffner’s tail clip and Eddy’s hot plate method. Recordings done at baseline and after drug administration at 60-, 120-, 150- and 180-minutes using stopwatch. Result: Using Haffner’s tail clip method, omeprazole showed an analgesic effect clinically at 60 min with high dose in Group III, but this was not statistically significant. With Eddy’s hot plate method, omeprazole showed an analgesic effect at a higher dose after 60 minutes of administration in Group III, which was statistically significant but the effect was not sustained. Conclusion: Although omeprazole showed analgesic effect at a high dose, the effect was not sustained. Further studies done for a longer duration are needed to establish its analgesic effect.