Background: Various microbes are associated with chronic wounds especially in the margin of ulcers. Both aerobic and anaerobic microbes are responsible for causing diabetic foot ulcers. Pseudomonas spp, E. coli, Proteus, S. aureus, and Enterococcus spp are the common pathogens isolated from diabetic foot infections. The present study was conducted for assessing Bacteriological Profile of Diabetic Foot Ulcer. Materials & Methods: 100 patients with diabetic foot ulcer were enrolled. The samples were collected after the wound was debrided. No antimicrobial agent or antiseptic was used in the wound before collection of the tissue specimen. The specimens were placed into sterile transport containers and sent to the microbiology laboratory for aerobic microbial culture as soon as possible. Cultures were processed following the standard procedures for tissue sample processing. Microbial growth plates were examined for growth on the following day. Microbiological profile was evaluated. All the results were recorded in Microsoft excel sheet and were subjected to statistical analysis using SPSS software. Results: While evaluating the microbiological profile, it was seen that E. coli and staphylococcus species were detected in 25 percent and 29 percent of the patients respectively. Klebsiella species and Enterococcus species were seen in 16 percent and 10 percent of the patients respectively. Proteus species, Enterobacter species, Serratia species and Pseudomonas species were seen in 5 percent, 8 percent, 3 percent and 4 percent of the cases respectively. Conclusion: Most common organisms among patients with diabetic foot ulcer was S. aureus. Monomicrobial infection is more prominent.