Background: Neonatal sepsis is major cause of neonatal morbidity and mortality worldwide. Blood culture and sepsis screening are currently used method, but their utility is limited due to delayed reporting and increased cost. Platelet indices are one such set of parameters which can be helpful in the future diagnosis of neonatal sepsis. This study was aimed to evaluate the significance of platelet indices as a marker of neonatal sepsis. Methods: Neonates with culture‑positive sepsis or clinical sepsisas per Centre for Disease Control (CDC) definition were categorized as cases and neonates initially suspected of having sepsis. Inborn and outborn babies admitted in NICU with clinical suspicion of neonatal sepsis were included. Babies with congenital and acquired causes of thrombocytopenia other than sepsis (e.g. autoimmune disorders of platelets, allo‑immune disorders of platelets) and congenital anomalies, congenital heart disease and Inborn Error of Metabolism were excluded from the study.All babies enrolled were investigated for blood culture, platelet indices (platelet count, MPV, PDW, PCT).Chi square test has been used for significantly and p value less than 0.05 has been taken to be significant.Results: A total of 120 babies were enrolled,platelet count has highest sensitivity (97.7%), NPV (96.5%) but PDW has highest specificity (77.7%), PPV (68.6%). All the test results were statistically significant with P value <0.001 except for PCT count which was not significant (p =0.18). Combined all makers platelet count + MPV have highest sensitivity (79.1%) and Platelet count + MPV + PDW + PCT have highest specificity (90.2%), PPV (76.6%) but Platelet count + PDW have highest NPV (81.9%). Conclusions: It may be concluded that platelet indices are sensitive markers to identify septic babies. The platelet count decreased with development of sepsis and PDW and MPV increased in septic babies. Thrombocytopenia was the most sensitive marker for culture‑positive sepsis, and the highest specificity of platelet indices was seen when all the platelet indices (platelet count + MPV + PDW + PC) or (MPV + PDW + PCT) were combined.