Context: Hemoglobinopathies are the most common heterogeneous group of monogenetic disorder in the world and its prevalence varies with geographical regions. India is developing country and many studies show a significant burden of hemoglobinopathies in India. Aim: The aim of the present study was to check the prevalence of various hemoglobinopathies in anemic subjects using high‑performance liquid chromatography (HPLC) method in Jabalpur region of Madhya Pradesh. Settings and design: The present study was conducted at the department of pathology on anemic patients referred from different outpatient department and Wards of the hospital and informed consent were taken from all participants. Subjects and methods: The present study included a total of 239 individuals. The HPLC test was performed using Bio‑Rad D‑10 analyzer during study period from October 2023 to March 2023. Results: Out of a total of 239 cases, we found 49 (20.50%) cases with abnormal hemoglobin fractions and (79.49%) cases free from hemoglobinopathies. Out of the total hemoglobinopathies detected 23 (46.93%) were male and 26 (53.06%) were female. The major abnormality detected was heterozygous sickle cell disorder with 34 (69.3%) cases, followed by Homozygous sickle cell disorders 6 (12.24%), Beta thalassemia trait 4 (8.16%), hereditary persistence of fetal hemoglobin 4 (8.16%),Hb J 2 (4.08%). Conclusions: In our study, we found a high prevalence of hemoglobinopathies among anemic subjects. The most common disorder detected was heterozygous sickle cell disorder. Most of the hemoglobinopathies found in our study could be accurately quantified by HPLC which is a rapid, sensitive, and reproducible method for the detection of different hemoglobinopathies.