Background: This study was conducted to compare Three-Port Versus Standard Four-Port Laparoscopic Cholecystectomy. Material and methods: 100 patients, ages 18 to 75, who were recommended for elective LC were prospectively enrolled for this study. Following a suitable level of general anesthesia, patients were randomized to undergo either 3-port (3-port group) or traditional LC (4-port group). Outcome was evaluated and compared. SPSS software was used for evaluation of results. Results:In this study, there were total 100 subjects who were divided into two groups of 50 each. Group 1 comprised of subjects undergoing 3-port cholecystectomy while the 2nd group comprised of subjects receiving 4-port cholecystectomy. AThe operating time was longer in group 2 (61.89 hours) as compared to the 1st group (45.26 hours). The analgesic tablet was required for 4 days in group 1 and for 5 days in group 2. The post operative stay was slightly longer in group 2.Conclusion: Comparing a 3-port laparoscopic cholecystectomy to a 4-port laparoscopic procedure, the former produced better clinical results.