Introduction: Menopause is a stage of women life indicating the cessation of the reproductive ability. Mostly the symptoms of menopause are started during 45 to 55 years. During Menopause ovarian follicular function is lost and blood estrogen and progesterone levels falls. Studies have found that the levels of serum calcium and vitamin-D are related to menopause in women. Calcium levels were found to be lower in post-menopause women when compared to pre-menopause women. The present study is focused on levels of calcium and vitamin-D in the postmenopausal women so that a healthy menopause can be observed in postmenopausal women. Materials & Methods: The present analysis study was carried out in 100 patients, out of these 50 women in reproductive period were included as control group. Other 50 women who have attained menopause were included as study group as inclusion and exclusion criteria. Care is taken for age matching among the two groups. The study has obtained approval from the Institute Ethics Committee of Arundathi Institute of Medical Sciences, Hyderabad, Telangana. Written informed consent has been obtained from all the participants of the study. The levels of serum calcium and vitamin D were analysed. Statistical analysis was done using IBM SPSS Statistics 20 package. p-value of <0.05 is considered as statistically significant and p-value of <0.005 is considered as statistically highly significant. The results were averaged as (mean ± standard deviation) for each parameter subgroups separately for Diabetics & Non-diabetics. Statistical analysis was done using IBM SPSS Statistics 20 package. Results: It is observed that the mean levels of vitamin-D are low in the post-menopause group when compared to pre-menopause group. Similarly the serum calcium was also found to be lower in post-menopause. This lower calcium and vitamin D levels increase the risk of osteoporosis. The mean levels of vitamin D in post-menopausal and pre menopausal groups are 17.5 and 30.72 respectively. Also, the levels of calcium in post-menopausal and pre menopausal groups are 7.25 and 9.116 respectively. Discussion: In the present study, the correlation between the levels of serum calcium, vitamin D are highly significant. In women after menopause, the significant decrease in serum calcium level specifies chances of high risk of negative calcium balance. Women after menopause shall be carefully monitored for levels of serum calcium, for lowering risk of bone resorption. Normal serum calcium levels do not confirm negative risk of osteoporosis, however low serum calcium suggests supplementation of calcium with follow up. It is advisable to monitor the levels of serum calcium level, vitamin D status and Bone Mineral Density for a happy postmenopausal life for women.