Dengue fever is the most prevalent arthropod-borne viral infection in human and is classified as major global health threat by the World Health Organisation (WHO).Platelet count is a simple but important accessory test which not only supports the diagnosis of dengue and also helps to monitor the disease progression.Platelet count of dengue positive cases were significantly lower than the dengue negative cases. While correlating dengue parameters with platelet count, thrombocytopenia was common in NS1 antigen associated cases than with cases positive for antibodies alone. Thrombocytopenia was much observed in secondary dengue infections than the primary infections. Aims & Objective:To evaluate diagnostic parameters inDengue febrile patients, who are ELISAconfirmed and tocorrelate the clinical condition with platelets count. Methodology: This prospective study conducted for a period of one year in Department of Microbiology, Gajra Raja Medical Collegeand J. A. Group of Hospitals (JAH), Gwalior, Madhya Pradesh.This study was performed among patients with febrile illness who were clinically symptomatic for dengue infection. A structured assessment form was used to obtain the clinical history regarding febrile illness including clinical symptoms and signs. Blood samples from clinically symptomatic OPD and IPD patients were collected. Results: A total number of 1,762 samples of clinically suspected dengue fever was processed for serological markers of dengue during the period of one year from January 2019 to December 2019.Among 1,762 samples,out of the 568 total positive cases of dengue, 439 (77.29%) showed thrombocytopenia. In 249 cases that were positive for NS1 Ag, thrombocytopenia was evident in 197 (79.12%) cases. and when IgM antibodies is found to be positive thrombocytopenia was noted in 151 out of 211 (71.56%) cases. when both the markers NS1 Ag + IgMAb was positive, thrombocytopenia was observed in 91 out of 108 (84.25%) cases. Conclusion: Currently dengue is globally the most important and rapidly spreading mosquito-borne viral disease.Out of these dengue specific parameters, platelet count is the only laboratory parameter performed in remote areas because of cost effectiveness and easy to perform that can support the diagnosis of dengue infection. Such predictions will help to decrease complication due to late treatment and initiate the preventive and control measures well in time for the containment of spread of the disease and contribute significantly to the clinical management which reduce morbidity and mortality in dengue infection.