Background: Diabetes mellitus is a metabolic disorder arises due to defect in insulin secretion, insulin action or both. This disease causes many complications which includes cardiovascular diseases. There are many causes of CVD like raised homocysteine level. Thus, this study was conducted to find out the homocysteine and blood sugar level in type 2 D.M. patients in relation to CVD risk factors. Materials and Methods: 300 Subjects were taken for the study in which 200 were diagnosed diabetes cases and 100 were healthy control subjects. Blood collected from subjects was centrifuged for estimation of serum homocysteine, vitamin B12, fasting blood sugar (FBS), total cholesterol (TC), triglyceride (TG), high density-lipoprotein cholesterol (HDL-c) was estimated by Vitros 5600 Integrated Chemistry Analyzer based on “Dry Slides Techniques. Low density lipoprotein cholesterol (LDL-c) and very low density lipoprotein cholesterol (VLDL-c) were estimated by Friedwald’s formula: LDL-c = TG- (HDL-c + VLDL-c) and VLDL-c = TG/5. Result: The homocysteine, glycemic parameters (FBS, PPBS, HbA1c) and lipid profile (Total cholesterol, triglycerides and VLDL-cholesterol) were significantly higher in T2DM patients at levels as compared to control subjects. Vitamin B12 was significantly lower in T2DM patients at 0.05 levels as compared to control subjects. Homocysteine is significant positively correlated (p-value<0.01) with HbA1c, TG and VLDL-cholesterol and significant negatively correlated (p- value<0.01) with HDL-cholesterol in T2DM patients. Conclusion: the genetic polymorphism may be the reason for increased homocystein level which causes insulin resistance and insulin resistance may be the reason for altered lipid profile.