Background: Pseudomonas aeruginosa is a gram negative bacterium that continues to be a major cause of opportunistic nosocomial infections, causing around 9-10% of hospital infections. The rapid increase of drug resistance in clinical isolates of this opportunistic human pathogen is a worldwide concern. Aims and Objectives: The study was undertaken to find out antimicrobial susceptibility pattern of Pseudomonas aeruginosa isolated from different clinical samples. Material and Methods: 106 samples of Pseudomonas aeruginosa isolates were included in this study. Identification was done by standard Microbiological procedures. Antibiotic susceptibility pattern was carried out by Vitek 2 automated system Results: Highest percentage of Pseudomonas aeruginosa were isolated from pus samples. Amikacin (64.15 %) was found to be more sensitive towards Pseudomonasaeruginosa followed by Cefoperazone/Sulbactum (61.32%), Ciprofloxacin(59.43%) and Meropenem(59.43%). Conclusions: Pseudomonas aeruginosa is one of the most important bacterial pathogen seriously contributing to the problem of healthcare associated infection. Maximum resistance in Pseudomonas aeruginosa isolates was seen against Ceftazidime,Piperacillin-Tazobactum and Levofloxacin.Hencethere is a need to emphasize the rational use of antimicrobials & strictly adhere to the concept of reserve drugs to minimize the misuse of available antimicrobials.