Original Articles
Comparison of concentration technique with direct microscopic examination for the detection of ova and cyst in stool sample | |
Veerendra Sen, Ritu Garg, Saurabh G Agarwal, Kuldeep Singh, Arun Raghuwansi, Rajdeep Paul | |
Introduction: Intestinal parasitic infections pose a significant health challenge globally, particularly in developing countries like India. Neglected tropical diseases, including parasitic infections, account for a substantial health burden. The prevalence of these infections varies regionally, with factors such as poor sanitation, hygiene practices, and immunological status influencing their spread. Protozoa and helminths are the primary agents causing these infections. Children in regions with inadequate sanitation are especially vulnerable, with potential implications for growth, anemia, and cognitive function. Various diagnostic methods are available, with direct wet mounts being common but limited by low sensitivity, particularly in cases of low parasite density. Materials and Methods: A prospective study was conducted on 100 stool samples over a 6-month period. Stool samples were processed using direct microscopy, including saline and iodine wet mounts, alongside concentration techniques (salt floatation and zinc sulphate floatation). The samples were analyzed for the presence of parasites, and demographic data were recorded. Results: Out of 100 samples, 20% tested positive for parasitic infection, with a higher prevalence in males (60%) compared to females (40%). The rural population showed a higher prevalence (70%) than the urban population (30%). Giardia lamblia cyst was the most common parasite (40%), followed by Entamoeba histolytica cyst (30%). The concentration techniques significantly increased the positivity rate compared to direct microscopy alone. Discussion: The study highlights the importance of concentration techniques in enhancing the sensitivity of parasite detection, especially in low-density infections. Factors such as gender, age group, and rural/urban residence influence the prevalence of parasitic infections. The zinc sulphate floatation technique demonstrated higher sensitivity compared to the saturated salt floatation method and direct microscopy. Conclusion: Parasitic infections remain a public health concern, particularly in developing nations. The study emphasizes the need for improved diagnostic methods, advocating for the inclusion of concentration techniques in routine stool examinations to enhance sensitivity. The findings underscore the regional variations and demographic factors influencing parasitic infection prevalence. Addressing these concerns requires a comprehensive approach, incorporating health education, sanitation measures, and targeted interventions. |
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