Background:A vital part of anaesthesia services is venepuncture. Children frequently undergo venepuncture (VP), a normal nursing technique that has the potential to cause pain, anxiety, fear, and distress if precautions are not taken. Pain from obtaining intravenous access can be eliminated with a quick technique employing a topical spray or cream. Regarding pharmacological therapies, a number of medications, including ethyl chloride, lignocaine, Eutectic mixture of local anesthetics (EMLA) cream and lidocaine, are shown better results in lowering pain. Objectives:To compare the effectiveness of EMLA cream and Ethyl Chloride spray in reduction of pain during venipuncture in paediatric population. Methodology:This randomised control study was conducted at Department of Anaesthesia in SRM medical college, Trichy. This study was conducted among paediatric age group six years and above up to twelve years of age group. They were randomly allocated in to two groups with n=30 each, in which group A received EMLA topical cream before venepuncture and Group B received Ethyl chloride spray before venepuncture. The effectiveness of anesthesia was assessed by VAS scores and heart rate changes during venepuncture. Data analysis was done using SPSS and continuous variables and categorical variables were interpreted using frequencies (mean±SD) and proportions (%). Results:Among the study participants the mean age among Group A participants was 9.13±1.85 years and the mean age of Group B participants was 9.47±1.907 years. The mean VAS scores (1.03) was lower in Group A compared with Group B the mean value of VAS scores was (1.53)and the p value was found to be statistically significant (p=<0.05). The heart rate changes was lower in Group A (82 bpm) compared with Group B (95 bpm) and the p value was found to be statistically significant (p=<0.05) Conclusion:EMLA cream was effective in reducing pain among children of age group 6-12 years undergoing intravenous injection.