Background:This study explores the interplay between testosterone levels, lifestyle behaviors, comorbidities, and physical performance in elderly men at a tertiary care center.Materials and Methods: A hospital-based cross-sectional study was conducted among 100 participants aged 60 and above. Data included lifestyle behaviors, comorbidities, Non-smokers comprised 71%, challenging the expected impact on testosterone. Alcohol users exhibited lower testosterone, contrary to assumptions. Surprisingly, non-exercisers had slightly higher SPPB scores. Chronic conditions like diabetes and hypertension showed nuanced associations with testosterone. BMI correlations were non-significant.Conclusion:Unexpected findings highlight the need for tailored health approaches in elderly populations. Holistic interventions should consider hormonal dynamics, lifestyle factors, and comorbidities, emphasizing individualized care for optimal aging.