Background: Infectious diseases are a major cause of mortality worldwide and exert a significant burden on the public health care system. The present study was conducted to assess serum procalcitonin in acute febrile illness as a predictor of bacterial infection. Materials & Methods: 70 patients with acute febrile illness of both genders were included. Serum Procalcitonin was measured using Finecare PCT rapid quantitative test. Levels of serum PCT > 0.5ng/ml were considered elevated. Results: Out of 70 patients, males were 40 and females were 30. 26 patients had TLC <11000 with PCT <0.5 and 12 had TLC >11000 with PCT <0.5. 21 patients had TLC <11000 with PCT >0.5 and 11 had TLC >11000 with PCT >0.5. The difference was non- significant (P> 0.05). Conclusion: Serum procalcitonin is a useful marker of bacterial infection.