Background: To understand the constantly changing pattern of microbial drug resistance it is crucial to have data analysis of various drug-bug susceptibility trends. In view of rising concern of antimicrobial resistance across the globe it is of prime importance to analyze local susceptibility trends and in turn to draw the local guidelines about the antimicrobial usage based on its outcome. This study aims to analyze the prevalent susceptibility patterns and identify the rate of resistant organisms among the bacterial culture positive isolates at the teaching tertiary care hospital in Gujarat in the year 2022. Total of 2754 clinical samples were sent to the bacteriology laboratory for the culture & sensitivity analysis during the period of one year, out of which 908(32.97%) were found to be culture positive where as 1846 (67.02%) were found to be culture negative. Out of 908 culture positive specimens the gram positive organisms were identified in 278(30.61%) isolates and gram negative organisms were identified in 630(69.38%) isolates. Further analysis of these isolates showed that among the gram negative strains, Escherichia coli (E.coli) was the predominant organism with total number of 291(32.04%) isolates, followed by Klebsiella spp with total number of 105(11.56%) isolates. Whereas among the gram positive strains, Staphylococcus aureus was the predominant organism with total number of 137(15.08%) isolates, followed by the Coagulase Negative Staphylococcus spp (CoNS) with total number of 78(8.59%) isolates. Inaddition this study also showed that there was significant number of isolates having resistant profiles including β lactamases, Methicillin resistant staphylococcus aureus (MRSA), and Carbapenemresistant Enterobacteriaceae (CRE). This analysis helps to understands the prevailing or developing resistant trends in comparison to the similar studies done in other parts of India and world