Background: The present study was undertaken for assessing pattern of drug resistance in sputum positive smear cases of pulmonary tuberculosis. Materials & methods: A total of 50 patients were included in this study. Informed consent was taken for getting the detailed history including previous anti tuberculosis treatment, for performing systemic physical examination and for required investigations. Two Sputum samples from each patient were collected in sterile containers and immediately sent for AFB smear microscopy. All sputum smears positive cases with previous history of treatment with first line antituberculosis drugs were instructed to collect sputum sample in a sterile, leak proof falcon tube. The samples were processed for Line Probe Assay. Results: H: Sensitive, R: Sensitive [isoniazid (H) and rifampicin (R)] was found to be present in 48 percent of the patients, while R: Sensitive, H: Resistant was found to be present in 24 percent of the patients. H: Sensitive, R: Resistant was found to be present in 8 percent of the patients while H: Sensitive, R: Sensitive was found to be present in 20 percent of the patients. Non-significant results were obtained while assessing the correlation of radiographic findings and H & R sensitivity. Conclusion: Line probe assay is a rapid and accurate tool for identification of drug resistance in pulmonary tuberculosis patients.