Background: Vitamin D, a fat-soluble vitamin derived from the effects of sunlight on the skin and from dietary sources, plays important roles in calcium–phosphate homeostasis and bone metabolism. The present study was conducted to assess serum 25-Hydroxyvitamin D levels of apparently healthy children aged 1-24 months. Material & Methods: The present retrospective study was carried out in the Department of Paediatrics over a period of one year. The retrospective data of 140 apparently healthy children aged 1 to 24 months were extracted and reported from the records. Serum 25(OH)D (vitamin D) was assayed. Data was analyzed using SPSS (version 20: IBM Corporation). The prevalence for each vitamin D groups as recommended by Endocrine Societywas determined. A p value of less than 0.05 was considered statistically significant. Results: Out of 140 children, 62(44.28%) children had deficient levels of Serum 25-Hydroxyvitamin D and 46 (32.85%) children had insufficient levels of Serum 25-Hydroxyvitamin D. Only 22.85% children had sufficient levels of Serum 25-Hydroxyvitamin D. The levels of Serum 25-Hydroxyvitamin D were deficient in 22.85%males and 21.42% females. The levels of Serum 25-Hydroxyvitamin D were insufficient in 18.57% males and 14.28% females. The levels of Serum 25-Hydroxyvitamin D were sufficient in 12.14% males and 10.71% females. Males were having deficient levels of Serum 25-Hydroxyvitamin D than females. In 1-6months children (14.28%) and 19-24months(12.85%) children, maximum children had deficient levels of Serum 25-Hyroxyvitamin D. In 7-12months (7.14%) children and 13-18 months children, maximum children had insufficient levels of Serum 25-Hyroxyvitamin D. Conclusion: The study concluded thatout of 140 children, 44.28% children had deficient levels of Serum 25-Hydroxyvitamin D and 32.85% children had insufficient levels of Serum 25-Hydroxyvitamin D. Males were having deficient levels of Serum 25-Hydroxyvitamin D than females. In 1-6months children and 19-24 months children, maximum children had deficient levels of Serum 25-Hyroxyvitamin D.