Aim: The main aim of this study is to know the ferrokinetic alterations like serum iron, TIBC, transferrin saturation and ferritin levels in absolute IDA & functional IDA patients with CKD. Methodology: A comparative ferrokinetic study was conducted in iron deficiency anaemia and anaemia of chronic renal disease subjects from Command Hospital Air Force Bangalore. Each subject gave an informed consent and this study was approved by the ethical and research committee of Command Hospital Air Force Bangalore to use human subjects in the research study. The patients voluntarily participated in the study.A total of 150 patients of CKD with age >18 years were included in our study, of which 40 (26.7%) cases were excluded because they did not satisfy inclusion criteria. Out of 110 subjects, 22 (20%) cases had absolute IDA, 60 (54.5%) had functional IDA and the remaining 28 (25.5%) cases had equivocal results. In all subjects, complete haemogram, serum iron, TIBC, transferrin saturation and serum ferritin were estimated. By using serum ferritin and TSAT, we classified patients into two groups absolute IDA and functional IDA & compared traditional indicators of iron status such as Hb, routine hemogram & correlated these two groups with EPO therapy, hypertension, diabetes status, haemodialysis & various stages of CKD in outpatient department patients. Results: In the present study, Results show that functional IDA is more common than absolute IDA in CKD patients. Absolute IDA was more common in females. No statistical significance in gender or age distribution was seen in the two subgroups of absolute and functional IDA. The severity of anaemia is significantly higher in patients with absolute IDA (Hb-6.78 g/dL ± 1.025) compared to patients with functional IDA (Hb-7.29g/dL ± 0.887) (p=0.0298). Mean values of MCV (70.5fl ± 5.4 and 84.7fl ± 4.5) (p <0.0001) and MCH (22.7pg ± 4.06 and 27.8pg ± 3.2) (p<0.0001) were significantly low in the absolute IDA group when compared to functional IDA group. There was no significant difference in the mean values of MCHC between the two groups (p=0.06).The differences in values of mean serum iron (30.15 µg/dL ± 9.5 versus 58 µg/dL ± 11.3; p<0.0001) and TIBC (484.8µg/dL ± 69.29 versus 325.23µg/dL ± 61.8; p<0.0001) were statistically significantly between absolute IDA group when compared to functional IDA group. The mean TSAT % was found to be significantly low in the absolute IDA group (6.60% ± 2.2) than compared to functional IDA cases (18.13% ± 6.6)(p<0.0001). The mean ferritin was found to be significantly low in the absolute IDA group (28.29ng/ml ± 12.6) when compared to functional IDA cases (336.6ng/ml ± 57) (p<0.0001). All of the patients with absolute IDA had microcytic hypochromic anaemia on PBS unlike patients with functional IDA who predominantly had normocytic normochromic anaemia. There were no statistically significant differences in the EPO requirement, prevalence of HTN or DM or stage of CKD between the two groups. Conclusion: Present study suggests that functional IDA is more common than absolute IDA in CKD patients. Serum iron, TIBC, TSAT, and serum ferritin are practical methods of quantifying iron deficiency in both IDA and CKD groups with sensitivity of serum ferritin and TSAT% together being 74.5%. These markers may help to take direct iron & EPO therapy in CKD patients.