Various solvent extract of leaves of Bryophyllum pinnatum were analyzed for antibacterial activity against selected species isolated from urine samples. To provide a scientific basis to treat UTIs, the extracts of leaves Bryophyllum pinnatum were subjected to antibacterial activity against selected bacterial species by well diffusion assay. MIC values were also determined to find minimum effective concentration. Methanolic extract show good antibacterial activity against highly resistant UTI isolates.