Background: A common skin disorder affecting a large population is vitiligo which shows a characteristic feature of selective melanocyte destruction. Literature data is scarce concerning the reliable biomarkers of insulin resistance in vitiligo subjects. Aim: The present study aimed to assess the role of serum desnutrin as a biomarker of insulin resistance in subjects having vitiligo vulgaris. Methods: The study assessed 90 subjects with vitiligo vulgaris from both genders and tested against 90 controls that were gender and age-matched. In all the subjects, a comprehensive cutaneous and general examination was done along with the assessment of serum desnutrin, fasting serum insulin, LDL, VLDL, HDL, triglycerides, cholesterol, and FBG (fasting blood glucose). Also, HOMA-IR (Homeostasis Model Assessment + insulin resistance) was done for all the participants. Results: The study results depicted a statistically significant difference for serum desnutrin, HOMA-IR, fasting insulin, FBG, and HDL in subjects with vitiligo vulgaris compared to the controls with p<0.001. Also, a negative correlation was seen in desnutrin and HOMA-IR, fasting insulin, VLDL, LDL, and FBS with p<0.05. A positive non-significant correlation was seen in HDL and desnutrin levels with p=0.06. Conclusion: The present study concludes that suppressed serum desnutrin levels are seen in subjects with vitiligo vulgaris secondary to increased serum insulin and glucose levels leading to insulin resistance and hyperlipidemia depicting desnutrin as a biomarker of insulin resistance in these subjects.