Background: Bacterial vaginosis (BV) is the most frequent vaginal infection. The present study was conducted to assess bacterial vaginosis (BV) in pregnant women. Materials & Methods: The present study was conducted over the period of two years from 2020 to 2021. 80 pregnant women with bacterial vaginosis (BV) were subjected to a detailed obstetric and per speculum examination. Vaginal and cervical swabs samples were obtained and sent to microbiology laboratory. All samples were processed for possible isolation and identification of pathogenic microorganisms. Results: There were 8 cases of BV in first trimester, 30 in second and 42 in third trimester. The difference was significant (P< 0.05). Staphylococcus aureus positivesamples was seen in 42, enterococcus positivein 7, group B Streptococci positive in 5, E. coli positive in 24, klebsiella positive in 6, proteus vulgaris positive in 3, candidapositive in 2. The difference was significant (P< 0.05). Conclusion: Common bacterial species in pregnant women with Bacterial vaginosis was staphylococcus aureus, enterococcus, group B Streptococci, E. coli, klebsiella, proteus vulgaris and candida.